FAQ for the 45HomeLab HL15

The total from the rear of the chassis to the middle fans is 10.5" so long as your GPU is not longer than that it will fit.

@LostFate makes a good point. If your power comes off the rear of the card you will not be able to fit a 10.5" card but if it comes off the side like a gaming GPU you should be fine.

Worse case you could get a low-profile fan and swap it out for the fans on the second row to give some extra clearance.